| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053100 |
| Chromosome: | chromosome 16 |
| Location: | 4596040 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687850 | PTK22 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype; Protein of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGAGGGCGACACATTAAGGGCGACGGG |
| Internal bar code: | AAGCCCGAACTAGCTTCCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 99.8655 |
| LEAP-Seq n confirming: | 4454 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAGGTGCTCGTCTGTCA |
| Suggested primer 2: | GGCACCCTACTGTCTCTGGA |