| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053104 |
| Chromosome: | chromosome_7 |
| Location: | 3386219 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g335100 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAAACGCAGGCGGACAGGGCGCCGGCAGC |
| Internal bar code: | TGACAAGCCATTACGTCGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1038 |
| LEAP-Seq percent confirming: | 99.7834 |
| LEAP-Seq n confirming: | 8753 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCGAAAGAGCTCACCGA |
| Suggested primer 2: | GGTGCCTTGTTTCTCATGGT |