| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053109 |
| Chromosome: | chromosome_2 |
| Location: | 2833109 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094600 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCCGGTCAGCCGCGCTGGGGCCGTTACGC |
| Internal bar code: | TGTATTACTGTCTTACCTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.4315 |
| LEAP-Seq n confirming: | 5597 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACGGGAGTAGCAGGCAG |
| Suggested primer 2: | GGGTTGTGGGTGGTGAATAG |