| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053116 |
| Chromosome: | chromosome 11 |
| Location: | 3049877 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478850 | (1 of 17) PF04755 - PAP_fibrillin (PAP_fibrillin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCCCGTCTCCCTTGCACACAAAATCAC |
| Internal bar code: | GACACGCGTGCACCCCACCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGATTGAGACCACCTA |
| Suggested primer 2: | CAAAGTGCGCATCGTAGAAA |