| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053152 |
| Chromosome: | chromosome 17 |
| Location: | 1027963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGGTGTTTGGCCTGCGCACCTGGAA |
| Internal bar code: | CGTCAGGCCCCGTTTTCGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 99.5479 |
| LEAP-Seq n confirming: | 11010 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAGATATATGCGATGCC |
| Suggested primer 2: | GATGCTAGTGAGGGAGCTGG |