| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053182 |
| Chromosome: | chromosome 14 |
| Location: | 1641576 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCCGAGAGTTGCTGGTCAAGTCAGCCT |
| Internal bar code: | GGAGTGACGCACTAACTCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 92.5723 |
| LEAP-Seq n confirming: | 6593 |
| LEAP-Seq n nonconfirming: | 529 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGGTTTTGGATGACAGT |
| Suggested primer 2: | AGGTGAGATGGCAGGTGAAC |