| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053184 |
| Chromosome: | chromosome 7 |
| Location: | 4730292 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345150 | (1 of 1) 2.3.1.108 - Alpha-tubulin N-acetyltransferase / Tubulin N-acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGGTCCGTGCTCCGTAGGCGGTATAT |
| Internal bar code: | CGGATTCGTCTTGCACGTCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 99.5825 |
| LEAP-Seq n confirming: | 954 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTGCTGAGTTGACCAC |
| Suggested primer 2: | GATGAAGCCAGCAGCCTAAG |