| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053186 |
| Chromosome: | chromosome 3 |
| Location: | 5288301 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183600 | (1 of 1) PF15375 - Domain of unknown function (DUF4602) (DUF4602) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGGATATCTGTCAGTACTGTACACCGA |
| Internal bar code: | GCCTGGCGTCGATACCTCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 99.4342 |
| LEAP-Seq n confirming: | 4218 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAACAGTGTGTGTGGGC |
| Suggested primer 2: | ATACCTGTATGCTGAGGCCG |