Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.053190 |
Chromosome: | chromosome_2 |
Location: | 5612821 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g109150 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCATCACCGCGTGTGCGGAGGACGGCACGT |
Internal bar code: | TTTATTGCTGTCTTTACTGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 890 |
LEAP-Seq percent confirming: | 99.5108 |
LEAP-Seq n confirming: | 1017 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTGGTGATATCTGCTGGGG |
Suggested primer 2: | GACTGCTGCGTCAACCTGTA |