| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053206 |
| Chromosome: | chromosome 7 |
| Location: | 3586090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336650 | MLH3 | DNA mismatch repair protein; (1 of 1) IPR003594//IPR020568 - Histidine kinase-like ATPase, C-terminal domain // Ribosomal protein S5 domain 2-type fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACGCGGTAGTCAATTGGAGACATGCCG |
| Internal bar code: | TCCCGATCAGCCGTCCGAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.6798 |
| LEAP-Seq n confirming: | 21792 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGTCCAAGAGCCTCAG |
| Suggested primer 2: | CGTCGAAGCTTTCTTCATCC |