| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053235 |
| Chromosome: | chromosome 17 |
| Location: | 1655242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708200 | HFO36,HFO26 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGTTTGTTAGAGGAAGGAGCAAAGAAA |
| Internal bar code: | GCGCGAAAACCGTCCTACACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 23.0118 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 455 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGCTGTTGCACCAATA |
| Suggested primer 2: | GGAGACACACAGCACGCTTA |