Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.053237 |
Chromosome: | chromosome_3 |
Location: | 5432409 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g185050 | FAP53 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAATACGCAGGACTCCTGCAACCGCATTG |
Internal bar code: | CCATTAAGCGATCCCACTACCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 529 |
LEAP-Seq percent confirming: | 99.6561 |
LEAP-Seq n confirming: | 2318 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGAAGGCGTTCATCCAGAG |
Suggested primer 2: | CACATGCTCACCAATCGAAC |