Insertion junction: LMJ.RY0402.053242_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.053242
Chromosome:chromosome 12
Location:6214271
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g536650 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACAGTAGTAGACCTTCAGCATATACTAT
Internal bar code:TCTAGAGGGAGAGCTTAGACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:15
LEAP-Seq percent confirming:99.0566
LEAP-Seq n confirming:315
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCAACTGCTTCATGTCC
Suggested primer 2:CACGCAAGGCTTGATCTACA