| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053281 |
| Chromosome: | chromosome 13 |
| Location: | 1188453 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569850 | AMT4,AMT1D | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCGAGCAAGGGTTTGTGGTCACGGCGG |
| Internal bar code: | ACGCGTGCCAGGGGCATGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.476 |
| LEAP-Seq n confirming: | 3417 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTGACTAGCAACCGATA |
| Suggested primer 2: | TGACAGTCTGGAGCAGGATG |