Insertion junction: LMJ.RY0402.053287_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.053287
Chromosome:chromosome 6
Location:3885795
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278178 (1 of 1) PF09495 - Protein of unknown function (DUF2462) (DUF2462) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCGCAACGAGGGCAGCTTGGCAGGTGCG
Internal bar code:CGAGCGCACAGCGACCACGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:312
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAACAAAACCCTCAAAAT
Suggested primer 2:GGCAGACAGTCAGCAAATGA