| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053320 |
| Chromosome: | chromosome 1 |
| Location: | 6457088 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045850 | (1 of 1) PF00665//PF07727 - Integrase core domain (rve) // Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTTGAGGCGGGCTGGCTAGATATTTG |
| Internal bar code: | ACCGAACTGAGAGGATTCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 953 |
| LEAP-Seq percent confirming: | 99.3056 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGTTGTCATTCGCACGC |
| Suggested primer 2: | TGCCAGTGAGCAGCTAGAGA |