| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053344 |
| Chromosome: | chromosome 16 |
| Location: | 757376 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690900 | RBL8 | rhomboid-like protein; (1 of 4) PTHR22936//PTHR22936:SF32 - RHOMBOID-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGCAACTTCCAAGTTATTTGCATACA |
| Internal bar code: | CATTCAGAGTCGAATGCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 99.4149 |
| LEAP-Seq n confirming: | 2209 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCCCTGCTGTCTACTTC |
| Suggested primer 2: | GAGCACGAGGAAAATAAGCG |