| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053352 |
| Chromosome: | chromosome 14 |
| Location: | 544602 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611552 | TGL22 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase; Putative triacylglycerol lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGATGCGGCCCAATTGTGCTTTTTGCGG |
| Internal bar code: | GAAGTACACGAGGGCGCTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 95.5117 |
| LEAP-Seq n confirming: | 1596 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAGATGCTGCTTTCGAC |
| Suggested primer 2: | GGGAAAGCATTATTGGGGTT |