| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053370 |
| Chromosome: | chromosome 17 |
| Location: | 3662385 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726600 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACCTCGGTCAGCTAAACATGGAACAAGA |
| Internal bar code: | TAAACTGTGGGCTCGCCACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 35 |
| LEAP-Seq percent confirming: | 99.5146 |
| LEAP-Seq n confirming: | 615 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCTCCACCAGCTTCCAG |
| Suggested primer 2: | ATCAGTCAGCAGGGAGCACT |