| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053390 |
| Chromosome: | chromosome_10 |
| Location: | 4630170 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g452950 | TRP1 | Transient receptor potential ion channel protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTTGGCTGCCCTGCCTCGCTTCGCCCCTG |
| Internal bar code: | CCAAATGCACCGGAGCGATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 91.2851 |
| LEAP-Seq n confirming: | 618 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGATGCTGATCAAGGAC |
| Suggested primer 2: | GATGAACAGGTTGAGCAGCA |