| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053404 |
| Chromosome: | scaffold 19 |
| Location: | 177392 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g751147 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCGTGCCTGCTCGAACGCCGATATAC |
| Internal bar code: | GTTGGGTGCCCGGACGGGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 15 |
| LEAP-Seq percent confirming: | 97.8956 |
| LEAP-Seq n confirming: | 1163 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACGATCTATTGAGGCTG |
| Suggested primer 2: | ATAATATGTGGGCGAGGTGC |