| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053406 |
| Chromosome: | chromosome 16 |
| Location: | 4021029 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670350 | (1 of 1) 2.7.1.81 - Hydroxylysine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAACGCAACCCCGGAAACGCCCAAACTT |
| Internal bar code: | GGAAGCGGCCTGATCTCAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 99.3929 |
| LEAP-Seq n confirming: | 4584 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACCGAAACCAACCAAGA |
| Suggested primer 2: | GGCTTGACGAGCTGTCCTAC |