Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.053419 |
Chromosome: | chromosome 13 |
Location: | 4014915 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g591073 | (1 of 2) PF00564 - PB1 domain (PB1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCAACCACACGGACCCTCCCGCCCCCC |
Internal bar code: | TGGCTGGCCGTCTGTACCTGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 147 |
LEAP-Seq percent confirming: | 22.6804 |
LEAP-Seq n confirming: | 22 |
LEAP-Seq n nonconfirming: | 75 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCACCATCACGCTAGACCT |
Suggested primer 2: | CGTCGTGTGTGTGTGTGTGT |