| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053424 |
| Chromosome: | chromosome 7 |
| Location: | 6057740 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355200 | ORC5 | Origin recognition complex subunit 5; (1 of 1) PTHR12705//PTHR12705:SF0 - ORIGIN RECOGNITION COMPLEX SUBUNIT 5 // ORIGIN RECOGNITION COMPLEX SUBUNIT 5 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAAGTAGTGAAAACTGCTGCTCAAACT |
| Internal bar code: | TGGGCAACCCTAGCTCGAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1248 |
| LEAP-Seq percent confirming: | 98.8912 |
| LEAP-Seq n confirming: | 3746 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTGGATAAGAGTGTGGG |
| Suggested primer 2: | ACTGTACCTGTGGGAGCGTT |