| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053434 |
| Chromosome: | chromosome 14 |
| Location: | 2326589 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623650 | ADH7 | Alcohol dehydrogenase; (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCCCTTACCTTCTCGGGGCGGCCGGAG |
| Internal bar code: | GGTCAACGGTCAGCAGGAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 99.6261 |
| LEAP-Seq n confirming: | 11191 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGATGTACGGTAACGGCA |
| Suggested primer 2: | AGCCCGATTCACGACATAAC |