| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053440 |
| Chromosome: | chromosome 6 |
| Location: | 3596107 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278122 | (1 of 1) PTHR10504:SF73 - PROTEIN C05C9.1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGTAAAAATGGAAGGCAGAAGCAGTA |
| Internal bar code: | AACTCATAGCGAATTGTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 99.4843 |
| LEAP-Seq n confirming: | 7523 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGATAGCTTCAGGCGT |
| Suggested primer 2: | AGGGTTTAGGTGGAGGTGCT |