| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053456 |
| Chromosome: | chromosome_8 |
| Location: | 4889526 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g384900 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCCCTGCTCCGCGCATGTGGCTGCATCTG |
| Internal bar code: | AAAAACCACTAGATAACAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 98.3582 |
| LEAP-Seq n confirming: | 659 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATTAGAGGGCTGAACG |
| Suggested primer 2: | AACCCAATGTGAAGGCAAAG |