| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053460 |
| Chromosome: | chromosome 9 |
| Location: | 4137914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393802 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGCTGCTGCTGTGGTGTGGGCCGGAAA |
| Internal bar code: | ATCCGGGTTGCATACGTTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.1949 |
| LEAP-Seq n confirming: | 4682 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAGAGGATGACAAGCG |
| Suggested primer 2: | ATGAGAGGGAGCAGCAGGTA |