| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053463 |
| Chromosome: | chromosome 2 |
| Location: | 5539492 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108550 | AOC3 | (1 of 4) PTHR11785//PTHR11785:SF367 - AMINO ACID TRANSPORTER // AMINO-ACID PERMEASE BAT1; Amino acid carrier | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGGCCCGCGGGGTGGGGAAGAAGCAG |
| Internal bar code: | ATTTATTACGTCCTAACCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 99.6696 |
| LEAP-Seq n confirming: | 905 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTAGCCTAACAGCCACC |
| Suggested primer 2: | ATTACTGTGGGTGGATGGGA |