Insertion junction: LMJ.RY0402.053496_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.053496
Chromosome:chromosome 11
Location:1049139
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467672 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACAGTCAAACTAACCCCCAGCTCCCGC
Internal bar code:TCCCCCCGTGACGGGCGTCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:99.5453
LEAP-Seq n confirming:10947
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGATCACGTGCTTGCTT
Suggested primer 2:CGAGGGGTGCTTTCATTTTA