| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053511 |
| Chromosome: | chromosome 16 |
| Location: | 1007730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649050 | CYG38 | (1 of 5) K12319 - guanylate cyclase soluble subunit beta (GUCY1B); Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTAAGGTGTGTGGGGGCGCTGGGGGGTA |
| Internal bar code: | TCCCCTGTGCGATTAGCGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 99.3062 |
| LEAP-Seq n confirming: | 16030 |
| LEAP-Seq n nonconfirming: | 112 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTCTTTCCACTGAGCC |
| Suggested primer 2: | GAGTTGGAGCTGGTGAGGAG |