| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053516 |
| Chromosome: | chromosome 16 |
| Location: | 2028941 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656950 | (1 of 1) PTHR13140:SF300 - PROTEIN TAG-278-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGACCTGGAGTCCCGCGTGGCAGCTGTG |
| Internal bar code: | CAGCGTGCCCAGGGTCGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 99.1501 |
| LEAP-Seq n confirming: | 350 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCAATCTATCCGTGCT |
| Suggested primer 2: | TTCTAGAATGGCAACCTCGG |