Insertion junction: LMJ.RY0402.053536_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.053536
Chromosome:chromosome 17
Location:4381699
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g731500 FAP394 Flagellar Associated Protein 394; (1 of 1) IPR000157//IPR011050 - Toll/interleukin-1 receptor homology (TIR) domain // Pectin lyase fold/virulence factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAACTGGCTTGGCCAGGAGTGGCAGGC
Internal bar code:GGGGCGAAATAATAGGAGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:110
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTACCCAACCTCCCACTG
Suggested primer 2:CAGAAAGCAACAGGAGAGGG