| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053561 |
| Chromosome: | chromosome 2 |
| Location: | 5489193 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108100 | (1 of 1) PTHR12147//PTHR12147:SF22 - FXNA-RELATED // ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGAGCAGGAGAAAGGGCACACAGCGTC |
| Internal bar code: | GAAAGTCACGCGCCTCGACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 99.5697 |
| LEAP-Seq n confirming: | 4397 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGCGTGT |
| Suggested primer 2: | TAACAGTGTGGGTATGCGGA |