| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053569 |
| Chromosome: | chromosome_2 |
| Location: | 8419082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141606 | DHC9 | Flagellar inner arm dynein heavy chain, dynein c (monomeric) | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCGGTGGGCTAGCGCATACCATGGTGAT |
| Internal bar code: | TATCTCTGAGGTGTTTACGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1173 |
| LEAP-Seq percent confirming: | 99.7797 |
| LEAP-Seq n confirming: | 13589 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGTGTGTGGAGGAAAG |
| Suggested primer 2: | GAGCACAAGGACTTTGAGGC |