| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053583 |
| Chromosome: | chromosome 1 |
| Location: | 5644270 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040200 | WNK2 | (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK); WNK protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCTTTTACCCTGCGTTTACCCTGAGA |
| Internal bar code: | CCTGGGCCGTGATCGCGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 98.2519 |
| LEAP-Seq n confirming: | 2473 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTGGGTAGGGCAATAAG |
| Suggested primer 2: | TCGACGAGTCGTGCAGTATC |