| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053607 |
| Chromosome: | chromosome 9 |
| Location: | 5740525 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401812 | (1 of 1) PF00580//PF13086//PF13087 - UvrD/REP helicase N-terminal domain (UvrD-helicase) // AAA domain (AAA_11) // AAA domain (AAA_12) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAACCATGGGGCGATGCATGGGTTAGT |
| Internal bar code: | CGTCTCGCGAAAGCCTGTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 99.041 |
| LEAP-Seq n confirming: | 1136 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTAAGAGCGAGCGAGAC |
| Suggested primer 2: | CCCTTACTCCTCACAGCTCG |