| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053612 |
| Chromosome: | chromosome 16 |
| Location: | 4573162 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688050 | (1 of 1) PTHR32091//PTHR32091:SF0 - FAMILY NOT NAMED // EUKARYOTIC INITIATION FACTOR 4B-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGCATGCGACAGTTGGAAAAGTTAACA |
| Internal bar code: | TCATTAATATCTTCGGGACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 99.6875 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTCGGTGACCTGGTGTT |
| Suggested primer 2: | TGGATAGTGGGCTAACTCGG |