| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053623 |
| Chromosome: | chromosome 2 |
| Location: | 4351513 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099350 | SRR8 | Scavenger receptor cysteine rich (SRCR) protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCACCATCGCTATCCCATCAGGGCATC |
| Internal bar code: | TGACCGAGATGATCGAGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 99.3667 |
| LEAP-Seq n confirming: | 10199 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATCCGAATTTCGCACCTG |
| Suggested primer 2: | TGATTGAAAAGCAAGCAACG |