| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053640 |
| Chromosome: | chromosome 13 |
| Location: | 1572427 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573150 | FPN5 | Purine 5'-nucleotidase; (1 of 1) PTHR12103:SF15 - PROTEIN Y71H10B.1, ISOFORM C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCATGTGAGGCGGTATGTGCTCATGTG |
| Internal bar code: | ACTTGTCAAGTGTTTTCCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 917 |
| LEAP-Seq percent confirming: | 99.8175 |
| LEAP-Seq n confirming: | 8752 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTCGTGTGTTCAGGTGC |
| Suggested primer 2: | CCTGAAGTAGCTGTAGGGCG |