Insertion junction: LMJ.RY0402.053673_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.053673
Chromosome:chromosome 4
Location:2056207
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g219050 SRR10 Scavenger receptor cysteine rich (SRCR) protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGATTTGGATGAGGCTGTACGAGTCTGTG
Internal bar code:TAATCTGCCCGCACGCATCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:559
LEAP-Seq percent confirming:99.5132
LEAP-Seq n confirming:4906
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TATCGTGCATACCCGACAGA
Suggested primer 2:CCGTAACCAGACAAAGCCAT