Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.053684 |
Chromosome: | chromosome 9 |
Location: | 4411700 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g395473 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGGCGAATACCGTACGCCACAGTAGA |
Internal bar code: | GGATATTACTGTGTCGTTGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1050 |
LEAP-Seq percent confirming: | 99.4421 |
LEAP-Seq n confirming: | 713 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAGTGTACTTGACGGCAGC |
Suggested primer 2: | CTCCGCACCATAGTAACCGT |