Insertion junction: LMJ.RY0402.053713_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.053713
Chromosome:chromosome 9
Location:6366068
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g406983 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGGCATTAGATTAGACCGGTGACGATG
Internal bar code:CCGTCGTTTTGTGTGATCTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:118
LEAP-Seq percent confirming:99.8047
LEAP-Seq n confirming:511
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACGTGACCCTGTTCCTC
Suggested primer 2:TAAACGGATGCGTGGTTACA