| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053715 |
| Chromosome: | chromosome 7 |
| Location: | 4347881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341251 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCTACATCACATGCAGGATTTCGGGCT |
| Internal bar code: | TGGTGCGTTGTGTATCCGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1179 |
| LEAP-Seq percent confirming: | 99.5035 |
| LEAP-Seq n confirming: | 13027 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAACAGCAACTTGACCAG |
| Suggested primer 2: | ACGCTTACCTGTATGGACGG |