Insertion junction: LMJ.RY0402.053717_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.053717
Chromosome:chromosome 16
Location:4749145
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g686750 PTA3 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCAGCTGGCCAAAGATGATGCCGCACA
Internal bar code:GGGGAGGGGATAGTCCGTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:750
LEAP-Seq percent confirming:99.3573
LEAP-Seq n confirming:773
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCATTGGTTTGGGGTCTA
Suggested primer 2:GCCACTCCGCTTAATATGGA