| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053725 |
| Chromosome: | chromosome 6 |
| Location: | 2782981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271100 | CDKG1 | Cyclin dependent kinase; (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACGTGCTGGTGGGCGTGCACTCTGGCA |
| Internal bar code: | GTGGGGTGTGAACGCCGTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 99.7041 |
| LEAP-Seq n confirming: | 10782 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACCAAGAAATGCTCAAT |
| Suggested primer 2: | CCACAGTGGACCTGGCTAAT |