| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053751 |
| Chromosome: | chromosome 12 |
| Location: | 3933332 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516300 | CGL154 | Conserved in the Green Lineage; (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGTGTGTGTGTGCGTGTTTGCGTGT |
| Internal bar code: | TCAGGTCATATGCTACGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 87.069 |
| LEAP-Seq n confirming: | 808 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGCTTGTCGTTTCTCTT |
| Suggested primer 2: | ACGATAGCACCCATCCACTC |