Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.053759 |
Chromosome: | chromosome 13 |
Location: | 1148480 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g569600 | (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTGGCGCCGCATGCAATCGAGCGCCT |
Internal bar code: | GGCGACCACAAGTGACCCATTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 130 |
LEAP-Seq percent confirming: | 65.5087 |
LEAP-Seq n confirming: | 7977 |
LEAP-Seq n nonconfirming: | 4200 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGACAGTCCGCTCTTCAC |
Suggested primer 2: | GCTTTCCCCACATGTTGTCT |