| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053768 |
| Chromosome: | chromosome 6 |
| Location: | 4266855 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278249 | AST5 | Putative aspartate aminotransferase; (1 of 1) PTHR11751//PTHR11751:SF33 - SUBGROUP I AMINOTRANSFERASE RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGGTTTTTAGGCTGGGCTGGCATCTAC |
| Internal bar code: | GCGGCGTTGCGGTGGCAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.4298 |
| LEAP-Seq n confirming: | 2267 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCTCGCTCTCCAGATCC |
| Suggested primer 2: | CTCATTCGCCAAGAGCTACC |