| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053783 |
| Chromosome: | chromosome_12 |
| Location: | 6202773 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536600 | FAP78 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCTCCCAGCACAGCGGGCGAAGTGAGTGG |
| Internal bar code: | GTCTGGCCCTCCGTGATCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 98.8372 |
| LEAP-Seq n confirming: | 340 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCGTGGAAGGTGTTGTC |
| Suggested primer 2: | ACACCGTCTTCTACAACCGC |